EXTRACTION AND STANDARDIZATION OF LUTEOLIN FROM SALVIA OFFICINALIS L. RAW MATERIAL: A REVIEW OF SCIENTIFIC LITERATURE

Abstract

In this literature review, a systematic analysis of scientific sources on the isolation (extraction) of luteolin belonging to the flavone class from the raw material Salvia officinalis L. (medicinal wormwood, "sage") and standardization at the preparative/analytical level was conducted. The review discussed the pharmacological significance of luteolin as a bioactive substance, its presence in Salvia officinalis (aglycone and glycosidic forms), the main technologies of extraction (massage, Soxhlet, reversible condensation under boiling, ultrasonic extraction, microwave extraction, liquid pressure extraction, eye solvent and green solvents), as well as the chain of enrichment and purification of luteolin (liquid-liquid distribution, SPE/C18, polyamide). Quantitative analysis based on HPLC-DAD, assessment of the total flavonoid content by the AlCl3 colorimetry method, as well as solutions according to the validation criteria (linearity, accuracy, reproducibility, resistance) according to ICH Q2 (R1) were systematized. The results showed the importance of distinguishing between 60-80% ethanol-water mixtures, hydrolyzed/non-hydrolyzed fractions, pH, and particle size regulation as optimal solvents for luteolin. Mechanisms for reducing industrial safety (GMP, residual solvents), series-to-series compatibility, and raw material variability were presented. As a result, a clear and practical protocol for luteolin was proposed: authentication - optimized extraction - purification - HPLC-DAD quantification - validation - stability - series control