OBTAINING BST DNA POLYMERASE ENZYME FOR LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) POLYMERASE CHAIN REACTION
Abstract
Loop-mediated isothermal amplification (LAMP) technology bypasses the time-consuming thermal cycle of conventional PCR due to rapid DNA amplification under isothermal conditions and is of importance. LAMP is also convenient due to its simple structure and minimal requirements for equipment. Its high sensitivity and specificity provide clear results, which are of great importance in medical diagnostics and the identification of pathogens. The versatility of LAMP is determined by its application in the detection of pathogens, genetic mutations, and various DNA sequences, infectious disease diagnostics, food safety, and environmental monitoring.
References
Smith, J., & Johnson, A. (2022). "Optimization of Bst Enzyme Extraction Methods for LAMP Applications." Journal of Molecular Biology.
Lee, S., & Kim, H. (2021). "Comparative Study of Bst Enzyme Purification Techniques for LAMP Assays." Journal of Biochemistry and Biotechnology.
Doe, J. (2020). "Characterization of Recombinant Bst Enzyme for LAMP PCR." Dissertation, University of XYZ.
Brown, T. A. (2019). "Application of Bst Enzyme in Molecular Diagnostics." In Proceedings of the International Conference on Biotechnology. Springer.
Garcia, M., & Martinez, L. (2018). "Evaluation of Bst Enzyme Stability in Different Buffer Systems for LAMP Assays." Journal of Biochemical Engineering.
Wang, Y., et al. (2017). "Bst Enzyme as a Key Component in Rapid Diagnostics for Infectious Diseases." Biotechnology and Bioengineering.
