ALLOKSAN CHAQIRGAN QANDLI DIABETDA OKSIDLOVCHI STRESS VA ANTIOKSIDANT HIMOYA TIZIMI O'RTASIDAGI BOG'LIQLIK: BIOKIMYOVIY VA PATOGENETIK JIHATLAR
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https://doi.org/10.5281/zenodo.20823260;
alloksan diabeti, oksidlovchi stress, antioksidant himoya, malon dialdegidi, superoksiddismutaza, katalaza, lipidlar peroksidlanishi, diabetik gepatopatiya, sitoliz, giperglikemiya.Abstrak
Ushbu tadqiqot alloksan yordamida modellashtrilgan eksperimental qandli diabet sharoitida jigar to'qimasidagi lipidlar peroksidlanishi (LPO) intensivligi, antioksidant fermentlar — superoksiddismutaza (SOD) va katalaza — faolligini hamda sitoliz markerlarini (ALT, AST) kompleks biokimyoviy tahlil asosida o'rganishga qaratilgan. Tajribalar vazni 180–220 g bo'lgan sog'lom oq kalamushlarda o'tkazildi. Hayvonlar nazorat (n=12) va alloksan-diabetik (n=12) guruhlarga ajratildi. Alloksan monohidrat 150 mg/kg dozasida qorin bo'shlig'iga yuborildi. 72 soatdan so'ng glikemiya ≥11 mmol/l bo'lgan hayvonlar tajribaga kiritildi. MDA miqdori TBK-usulida, SOD faolligi nitrosiniy tetrazoliy ingibitsiyasi, katalaza — ammoniy molibdat kompleksi, ALT/AST — Reytman–Frenkel usulida aniqlandi. Diabet guruhida MDA darajasi 3,93 baravar oshdi (1,24±0,09 dan 4,87±0,21 nmol/mg oqsilgacha, p<0,001). SOD faolligi 50,0% pasaydi (18,6±0,8 dan 9,3±0,6 U/mg oqsilgacha, p<0,001), katalaza faolligi 53,2% kamaydi (42,3±2,1 dan 19,8±1,4 mmol H₂O₂/min/mg oqsilgacha, p<0,001). ALT va AST faolligi mos ravishda 2,98 va 3,13 barobarga oshib, kuchli sitoliz sindromini ko'rsatdi.Iqtiboslar
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