O‘SIMLIK XOMASHYOSIDAN AJRATIB OLINGAN BIOLOGIK FAOL FLAVONOIDLAR KOMPLEKSINI MODIFIKATSIYALASH VA ULARNING VIRUSLARGA QARSHI FAOLLIGINI IN SILICO HAMDA IN VITRO USULLARDA BAHOLASH
DOI:
https://doi.org/10.5281/zenodo.21484067Keywords:
Flavonoidlar, modifikatsiya, in silico, molekulyar doking, in vitro, antivirual faollik, neyraminidaza, SARS-CoV-2 Mpro, Vero E6, terapevtik indeks.Abstract
Ushbu ilmiy tezisda Glycyrrhiza glabra L. va Sophora japonica L. o‘simlik xomashyosidan ajratib olingan flavonoidlar majmuasini (ksantoxumol, kversetin, rutin) kimyoviy modifikatsiyalash va ularning viruslarga qarshi faolligini kompleks baholash natijalari keltirilgan. Flavonoidlar strukturasi yarim sintetik asillash hamda glikozillash reaksiyalari orqali o‘zgartirilib, ularning biofoydalanish darajasi va fermentativ retsipiyentlar bilan bog‘lanish afilligi oshirildi. In silico molekulyar doking tahlillari yordamida hosilalarning Gripp A (H1N1) neyraminidazasi hamda SARS-CoV-2 Mpro proteazasi bilan bog‘lanish energiyalari (ΔG) hisoblab chiqildi. In vitro tajribalarida Vero E6 va MDCK hujayra kulturalarida birikmalarning sitotoksikligi (CC50) va ingibirlash konsentratsiyasi (IC50) aniqlanib, terapevtik indekslari (TI) hisoblandi. Olingan natijalar modifikatsiyalangan flavonoid hosilalarining virus ingibitorlari sifatida yuqori samaradorlikka ega ekanligini tasdiqlaydi.References
Havsteen, B. H. (2002). The biochemistry and medical significance of the flavonoids. Pharmacology & Therapeutics, 96(2-3), 67-202.
Xiao, J. (2017). Dietary flavonoid aglycones and their glycosides: Which show better biological benefits?. Critical Reviews in Food Science and Nutrition, 57(9), 1874-1905.
Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal, 2013, 1-16.
Zu, G., Zhang, R., et al. (2012). Ultrasound-assisted extraction of flavonoids from Sophora japonica L. Industrial Crops and Products, 36(1), 161-167.
Wright, J. S., et al. (2001). Predicting the antioxidant activity of curcumin in silico. Journal of the American Chemical Society, 123(6), 1173-1179.
Trott, O., & Olson, A. J. (2010). AutoDock Vina: improving the speed and accuracy of docking. Journal of Computational Chemistry, 31(2), 455-461.
Jin, Z., Du, X., et al. (2020). Structure of Mpro from SARS-CoV-2 enables rapid development of antiviral drugs. Nature, 582(7811), 289-293.
Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
Pizzorno, A., et al. (2021). In vitro evaluation of antiviral activity against SARS-CoV-2. Antiviral Research, 189, 105055, 1-9.
Chen, L., et al. (2020). Binding interaction of quercetin derivatives with SARS-CoV-2 main protease. Journal of Biomolecular Structure and Dynamics, 39(14), 5120-5128.
Zakaryan, H., et al. (2017). Flavonoids: promising antiviral agents against acute respiratory infections. Archives of Virology, 162(9), 2539-2551.
Kaul, T. N., et al. (1985). Antiviral effect of flavonoids on human viruses. Journal of Medical Virology, 15(1), 71-79.
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