O‘SIMLIK XOMASHYOSIDAN AJRATIB OLINGAN BIOLOGIK FAOL FLAVONOIDLAR KOMPLEKSINI MODIFIKATSIYALASH VA ULARNING VIRUSLARGA QARSHI FAOLLIGINI IN SILICO HAMDA IN VITRO USULLARDA BAHOLASH
DOI:
https://doi.org/10.5281/zenodo.21484067Ключевые слова:
Flavonoidlar, modifikatsiya, in silico, molekulyar doking, in vitro, antivirual faollik, neyraminidaza, SARS-CoV-2 Mpro, Vero E6, terapevtik indeks.Аннотация
Ushbu ilmiy tezisda Glycyrrhiza glabra L. va Sophora japonica L. o‘simlik xomashyosidan ajratib olingan flavonoidlar majmuasini (ksantoxumol, kversetin, rutin) kimyoviy modifikatsiyalash va ularning viruslarga qarshi faolligini kompleks baholash natijalari keltirilgan. Flavonoidlar strukturasi yarim sintetik asillash hamda glikozillash reaksiyalari orqali o‘zgartirilib, ularning biofoydalanish darajasi va fermentativ retsipiyentlar bilan bog‘lanish afilligi oshirildi. In silico molekulyar doking tahlillari yordamida hosilalarning Gripp A (H1N1) neyraminidazasi hamda SARS-CoV-2 Mpro proteazasi bilan bog‘lanish energiyalari (ΔG) hisoblab chiqildi. In vitro tajribalarida Vero E6 va MDCK hujayra kulturalarida birikmalarning sitotoksikligi (CC50) va ingibirlash konsentratsiyasi (IC50) aniqlanib, terapevtik indekslari (TI) hisoblandi. Olingan natijalar modifikatsiyalangan flavonoid hosilalarining virus ingibitorlari sifatida yuqori samaradorlikka ega ekanligini tasdiqlaydi.Библиографические ссылки
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