DEVELOPMENT OF METHODS FOR QUALITATIVE AND QUANTITATIVE DETERMINATION OF ASCORBIC ACID IN DIETARY SUPPLEMENTS IN THE FORM OF GUMMY PASTILLES

Abstract:

This article presents the results of research on the development of methods for the identification and quantitative determination of ascorbic acid in dietary supplements in the form of gummy pastilles. Given the complex matrix of the gummy dosage form, which contains gelling agents and sugars, High-Performance Liquid Chromatography (HPLC) with UV detection was selected for the analysis. Titrimetric determination using sodium 2,6-dichloroindophenolate was used as a comparative method. It was established that the developed HPLC method is characterized by high selectivity and reproducibility. The content of ascorbic acid in one gummy pastille ranged from 10.1 to 10.3 mg. The results obtained confirm the feasibility of using the proposed method for the quality control of dietary supplements in gummy forms.