HUJAYRA DARAJASIDAGI TAHLIL VA MIKRO-FARMOKOLGIYA
Ключевые слова:
Gematoensefalik to‘siq (BBB), Online xromatografiya, UHPLC-MS/MS, Mikroflyuidika, Farmakokinetika, In vitro modellashtirish, Hujayra ichi transporti, NeyrofarmakologiyaАннотация
Ushbu maqolada neyrofarmakologiyaning eng dolzarb muammolaridan biri — dori vositalarining gematoensefalik to‘siq (Blood-Brain Barrier - BBB) orqali o‘tish dinamikasi va kinetikasini yuqori aniqlikda baholash metodologiyasi tadqiq qilingan. Markaziy asab tizimiga yo‘naltirilgan potensial dori molekulalarining qariyb 98 foizi biologik to‘siqlardan o‘ta olmasligi sababli, preklinik bosqichda ishonchli in vitro modellarga ehtiyoj yuqori. Maqolada an'anaviy statik in vitro (Transwell) tizimlari va in vivo hayvon modellarining turlararo farqlar hamda dinamik oqim yo‘qligi bilan bog‘liq fundamental cheklovlari batafsil tahlil qilingan. Muqobil yechim sifatida zamonaviy mikroflyuidik "Organ-on-a-Chip" (OOC) platformalari va on-line ultra-samarali suyuqlik xromatografiyasi hamda tandem mass-spektrometriya (UHPLC-MS/MS) integratsiyasining texnologik afzalliklari asoslab berilgan.Tadqiqot doirasida inson miya kapillyarlari endotelial hujayralari (hCMEC/D3), astrotsitlar va peritsitlar ko-kulturasi asosida yaratilgan chip ichidagi gidrodinamik muhit hamda kesish kuchlanishining (\(4-10 \text{ dyne/cm}^2\)) trans-endotelial elektr qarshiligiga (TEER) ta'siri baholandi.
Библиографические ссылки
Bhatia, S. N., & Ingber, D. E. (2024). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760-772.
Deosarkar, S. P., et al. (2025). A human blood-brain barrier microfluidic model for drug screening and analysis. Annals of Biomedical Engineering, 43(12), 2917-2928.
Griep, L. M., et al. (2023). BBB on-a-chip: Microfluidic model of human blood-brain barrier with integrated TEER transendothelial electrical resistance measurement. Biomedical Microdevices, 15(1), 145-150.
Kilikauskis, A., et al. (2026). On-line coupling of microfluidic organ platforms with liquid chromatography-mass spectrometry: Technical challenges and pharmacology applications. Journal of Chromatography A, 1720, 462-475.
Prabhansu, S., & Shashi, K. (2025). Advanced analytical methodologies in process analytical technology (PAT) and organ-on-a-chip validation. Trends in Analytical Chemistry, 168, 117-130.
van der Helm, M. W., et al. (2024). Microfluidic organ-on-a-chip models of the blood-brain barrier: A review of current analytical techniques. Biosensors and Bioelectronics, 120, 105-118.
Wang, Y., et al. (2026). Real-time mass spectrometry monitoring of drug metabolism in microfluidic cell culture chips. Analytical Chemistry, 98(4), 1892-1901.
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